TranscriptionTumorLaboratory2019

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Transcriptional regulation during tumoral transformation

The Transcription and Tumor Laboratory
Lab Members
Martin Teichmann
Professor
Director of the Doctoral School of
Life Sciences and Health
email: Martin.Teichmann@inserm.fr
phone: ++33-5-5757-4647
 
Hélène Dumay-Odelot
Associate Professor
email: Helene.Dumay-Odelot@u-bordeaux.fr
phone: ++33-5-5757-5691
 
Francis Sagliocco
Associate Professor
Coordinator of the Master of Cellular and Molecular Genetics
email: Francis.Sagliocco@u-bordeaux.fr
phone: ++33-5-5757-9504
Angélique Alonso-Marrau
Technician
email: Angelique.Alonso-Marrau@u-bordeaux.fr
phone: ++33-5-5757-9504
Teaching
Our Team members are participating in teaching modules of the bachelor degree and of the Masters of Genetics (GMC), Cellular Biology (BCPP), Cancer Biology and Biochemistry of the University of Bordeaux.
For more detailed information, please contact the laboratory.
Research
We are interested in dissecting mechanisms that are altered in transcription regulation during the process of tumoral transformation. For that purpose, we employ defined cellular model systems of stepwise transformation of human cells. We are in addition interested in determining the roles of RNA polymerase III in the development of leukodystrophies.
Selected Publications

TFIIIC binding to Alu elements controls gene expression via chromatin looping and histone acetylation.

Roberto Ferrari, Lara Isabel de Llobet Cucalon, Chiara Di Vona, François Le Dilly, Enrique Vidal, Antonios Lioutas, Javier Quilez Oliete, Laura Jochem, Erin Cutts, Giorgio Dieci, Alessandro Vannini, Martin Teichmann,  Susana de la Luna, Miguel Beato.

doi: https://doi.org/10.1101/455733

https://www.biorxiv.org/content/10.1101/455733v3

Accepted for publication in Molecular Cell

The hRPC62 subunit of human RNA polymerase III displays helicase activity.

El Ayoubi L, Dumay-Odelot H, Chernev A, Boissier F, Minvielle-Sébastia L, Urlaub H, Fribourg S, Teichmann M.

Nucleic Acids Res. 2019 Sep 16. pii: gkz788. doi: 10.1093/nar/gkz788.

https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz788/5569986?searchresult=1

 

Fusion-mediated genomic instability promotes aneuploidy patterns that resemble human tumors.

Delespaul, L, Merle , C, Lesluyes, T, Lagarde, P, Le Guellec, S, Pérot, G, Baud, J, Prochazkova-Carlotti, M,  Danet, C, Fèvre, M, Rousseau, B, Durrieu-Gaillard, S, Teichmann, M, Coindre, JM, Lartigue, L, Chibon, F. Oncogene. 2019 Jul 3. doi: 10.1038/s41388-019-0859-6. [Epub ahead of print]

https://www.nature.com/articles/s41388-019-0859-6

 

Leukodystrophy-associated POLR3A mutations down-regulate the RNA polymerase III transcript and important regulatory RNA BC200.

Karine Choquet, K, Forget, D, Meloche, E, Dicaire, MJ, Bernard, G, Vanderver, A, Schiffmann, R, Fabian, M, Teichmann, M, Coulombe, B, Brais, B, and Kleinman, C. J Biol Chem. 2019 May 3;294(18):7445-7459. doi: 10.1074/jbc.RA118.006271.

http://www.jbc.org/content/early/2019/03/21/jbc.RA118.006271

CRISPR/Cas9 genome editing induces megabase-scale chromosomal truncations.

Cullot, G, Boutin, J, Toutain, J, Prat, F, Pennamen, P, Rooryck, C, Teichmann, M, Rousseau, E, Lamrissi-Garcia, I, Guyonnet-Duperat, V, Bibeyran, A, Lalanne, M, Prouzet-Mauléon, V, Turcq, B, Ged, C, Blouin, JM, Richard, E, Dabernat, S, Moreau-Gaudry, F, Bedel, A.

Nat Commun. 2019 Mar 8;10(1):1136. doi: 10.1038/s41467-019-09006-2.

https://doi.org/10.1038/s41467-019-09006-2

Effects on prostate cancer cells of targeting RNA polymerase III.
Petrie1, JL, Swan, C, Ingram, RM, Frame, FM, Collins, AT, Dumay-Odelot, H, Teichmann, M, Maitland, NJ, White, RJ.

Nucleic Acids Res. 2019 May 7;47(8):3937-3956. doi: 10.1093/nar/gkz128.

https://doi.org/10.1093/nar/gkz128

Mutation in POLR3K causes hypomyelinating leukodystrophy and abnormal ribosomal RNA regulation.

Dorboz I, Dumay-Odelot H, Boussaid K, Bouyacoub Y, Barreau P, Samaan S, Jmel H, Eymard-Pierre E, Cances C, Bar C, Poulat AL, Rousselle C, Renaldo F, Elmaleh-Bergès M, Teichmann M, Boespflug-Tanguy O.

Neurol Genet. 2018 Dec 3;4(6):e289.

https://doi.org/10.1212/NXG.0000000000000289

Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements.

Durrieu-Gaillard S, Dumay-Odelot H, Boldina G, Tourasse NJ, Allard D, André F, Macari F, Choquet A, Lagarde P, Drutel G, Leste-Lasserre T, Petitet M, Lesluyes T, Lartigue-Faustin L, Dupuy JW, Chibon F, Roeder RG, Joubert D, Vagner S, Teichmann M.

Cell Cycle. 2018;17(5):605-615.

https://doi.org/10.1080/15384101.2017.1405881

Absence of neurological abnormalities in mice homozygous for the Polr3a G672E hypomyelinating leukodystrophy mutation.

Choquet K, Yang S, Moir RD, Forget D, Larivière R, Bouchard A, Poitras C, Sgarioto N, Dicaire MJ, Noohi F, Kennedy TE, Rochford J, Bernard G, Teichmann M, Coulombe B, Willis IM, Kleinman CL, Brais B.

Mol Brain. 2017 Apr 13;10(1):13.

https://doi.org/10.1186/s13041-017-0294-y

TRM6/61 connects PKCα with translational control through tRNAi(Met) stabilization: impact on tumorigenesis.

Macari F, El-Houfi Y, Boldina G, Xu H, Khoury-Hanna S, Ollier J, Yazdani L, Zheng G, Bièche I, Legrand N, Paulet D, Durrieu S, Byström A, Delbecq S, Lapeyre B, Bauchet L, Pannequin J, Hollande F, Pan T, Teichmann M, Vagner S, David A, Choquet A, Joubert D.

Oncogene. 2016 Apr 7;35(14):1785-96.

https://doi.org/10.1038/onc.2015.244

Structural analysis of human RPC32β-RPC62 complex.

Boissier F, Dumay-Odelot H, Teichmann M, Fribourg S.

J Struct Biol. 2015 Dec;192(3):313-319.

https://doi.org/10.1016/j.jsb.2015.09.004

​​

Activation and repression by oncogenic MYC shape tumour-specific gene expression profiles.

Walz S, Lorenzin F, Morton J, Wiese KE, von Eyss B, Herold S, Rycak L, Dumay-Odelot H, Karim S, Bartkuhn M, Roels F, Wüstefeld T, Fischer M, Teichmann M, Zender L, Wei CL, Sansom O, Wolf E, Eilers M.

Nature. 2014 Jul 24;511(7510):483-7.

https://doi.org/10.1038/nature13473

Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.

Dumay-Odelot H, Durrieu-Gaillard S, El Ayoubi L, Parrot C, Teichmann M.

Transcription. 2014;5(1):e27526.

https://doi.org/10.4161/trns.27526

RNA polymerase III transcription - regulated by chromatin structure and regulator of nuclear chromatin organization.

Pascali C, Teichmann M.

Subcell Biochem. 2013;61:261-87.

https://doi.org/10.1007/978-94-007-4525-4_12

Recessive mutations in POLR3B, encoding the second largest subunit of Pol III, cause a rare hypomyelinating leukodystrophy.

Tétreault M, Choquet K, Orcesi S, Tonduti D, Balottin U, Teichmann M, Fribourg S, Schiffmann R, Brais B, Vanderver A, Bernard G.

Am J Hum Genet. 2011 Nov 11;89(5):652-5.

https://doi.org/10.1016/j.ajhg.2011.10.006

Mutations of POLR3A encoding a catalytic subunit of RNA polymerase Pol III cause a recessive hypomyelinating leukodystrophy.

Bernard G, Chouery E, Putorti ML, Tétreault M, Takanohashi A, Carosso G, Clément I, Boespflug-Tanguy O, Rodriguez D, Delague V, Abou Ghoch J, Jalkh N, Dorboz I, Fribourg S, Teichmann M, Megarbane A, Schiffmann R, Vanderver A, Brais B.

Am J Hum Genet. 2011 Sep 9;89(3):415-23.

https://doi.org/10.1016/j.ajhg.2011.07.014

Widespread occurrence of non-canonical transcription termination by human RNA polymerase III.

Orioli A, Pascali C, Quartararo J, Diebel KW, Praz V, Romascano D, Percudani R, van Dyk LF, Hernandez N, Teichmann M, Dieci G.

Nucleic Acids Res. 2011 Jul;39(13):5499-512.

https://doi.org/10.1093/nar/gkr074

Structure-function analysis of hRPC62 provides insights into RNA polymerase III transcription initiation.

Lefèvre S, Dumay-Odelot H, El-Ayoubi L, Budd A, Legrand P, Pinaud N, Teichmann M, Fribourg S.

Nat Struct Mol Biol. 2011 Mar;18(3):352-8.

https://doi.org/10.1038/nsmb.1996

General transcription factors and subunits of RNA polymerase III: Paralogs for promoter- and cell type-specific transcription in multicellular eukaryotes.

Teichmann M, Dieci G, Pascali C, Boldina G.

Transcription. 2010 Nov;1(3):130-135.

https://www.researchgate.net/publication/49841510_General_transcription_factors_and_subunits_of_RNA_polymerase_III

Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.

Dumay-Odelot H, Durrieu-Gaillard S, Da Silva D, Roeder RG, Teichmann M.

Cell Cycle. 2010 Sep 15;9(18):3687-99.

https://www.researchgate.net/publication/47300625_Cell_growth-_and_differentiation-dependent_regulation_of_RNA_polymerase_III_transcription

Two isoforms of human RNA polymerase III with specific functions in cell growth and transformation.

Haurie V, Durrieu-Gaillard S, Dumay-Odelot H, Da Silva D, Rey C, Prochazkova M, Roeder RG, Besser D, Teichmann M.

Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4176-81. https://doi.org/10.1073/pnas.0914980107

PTEN represses RNA polymerase III-dependent transcription by targeting the TFIIIB complex.

Woiwode A, Johnson SA, Zhong S, Zhang C, Roeder RG, Teichmann M, Johnson DL.

Mol Cell Biol. 2008 Jun;28(12):4204-14.

https://doi.org/10.1128/MCB.01912-07

The expanding RNA polymerase III transcriptome.

Dieci G, Fiorino G, Castelnuovo M, Teichmann M, Pagano A.

Trends Genet. 2007 Dec;23(12):614-22.

https://www.ncbi.nlm.nih.gov/pubmed/17977614

Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC.

Dumay-Odelot H, Marck C, Durrieu-Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, Pflieger A, Teichmann M.

J Biol Chem. 2007 Jun 8;282(23):17179-89.

http://www.jbc.org/content/282/23/17179.full

Nuclear particles containing RNA polymerase III complexes associated with the junctional plaque protein plakophilin 2.

Mertens C, Hofmann I, Wang Z, Teichmann M, Sepehri Chong S, Schnölzer M, Franke WW.

Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7795-800.

https://www.researchgate.net/publication/11924398_Nuclear_particles_containing_RNA_polymerase_III_complexes_associated_with_the_junctional_plaque_protein_plakophilin_2

Spermiogenesis deficiency in mice lacking the Trf2 gene.

Zhang D, Penttila TL, Morris PL, Teichmann M, Roeder RG.

Science. 2001 May 11;292(5519):1153-5.

https://www.ncbi.nlm.nih.gov/pubmed/11352070

A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements.

Teichmann M, Wang Z, Roeder RG.

Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14200-5.

https://www.pnas.org/content/97/26/14200.long

Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells.

Teichmann M, Wang Z, Martinez E, Tjernberg A, Zhang D, Vollmer F, Chait BT, Roeder RG.

Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13720-5.

https://www.pnas.org/content/96/24/13720

 

Functional interchangeability of TFIIIB components from yeast and human cells in vitro.

Teichmann M, Dieci G, Huet J, Rüth J, Sentenac A, Seifart KH.

EMBO J. 1997 Aug 1;16(15):4708-16.

http://emboj.embopress.org/content/16/15/4708

Physical separation of two different forms of human TFIIIB active in the transcription of the U6 or the VAI gene in vitro.

Teichmann M, Seifart KH.

EMBO J. 1995 Dec 1;14(23):5974-83.

https://www.embopress.org/doi/pdf/10.1002/j.1460-2075.1995.tb00286.x

Address
University of Bordeaux
Inserm U1212 - CNRS UMR 5320
146, rue Léo Saignat
Bâtiment 3A, 1st floor
33076 Bordeaux
FRANCE
Funding bodies
Ligue contre le Cancer:
                      - "équipe labellisée" from 2011 to 2015
 
INCa:
ANR:
ARC:
ODYSSEA:
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